A Practical Guide to Basic Laboratory AndrologyLars Björndahl, David Mortimer, Christopher L. R. Barratt, Jose Antonio Castilla, Roelof Menkveld, Ulrik Kvist, Juan G. Alvarez, Trine B. Haugen Cambridge University Press, 1 abr 2010 This practical, extensively illustrated handbook covers the procedures that are undertaken in andrology and ART laboratories to analyse and assess male-factor infertility, and to prepare spermatozoa for use in assisted conception therapy. The content is presented as brief, authoritative overviews of the relevant biological background for each area, plus detailed, step-by-step descriptions of the relevant analytical procedures. Each technical section includes pertinent quality control considerations, as well as the optimum presentation of results. In addition to the comprehensive 'basic' semen analysis, incorporating careful analysis of sperm morphology, the handbook provides established techniques for the use of computer-aided sperm analysis and sperm functional assessment. Throughout the handbook the interpretation of laboratory results in the clinical context is highlighted, and safe laboratory practice is emphasized. It is an invaluable resource to all scientists and technicians who perform diagnostic testing for male-factor infertility. |
Índice
1 | |
Basic semen analysis | 33 |
Extended semen analysis | 77 |
Sperm function tests | 113 |
Tests of spermcervical mucus interaction | 147 |
Sperm preparation | 167 |
Sperm cryobanking | 189 |
Preparation of surgically retrieved spermatozoa | 219 |
Reproductive toxicology | 257 |
Equipment required for a basic andrology laboratory | 277 |
Supply companies | 287 |
Home testing | 295 |
Comparison of replicate counts | 305 |
Safety | 313 |
326 | |
332 | |
Otras ediciones - Ver todo
A Practical Guide to Basic Laboratory Andrology Lars Björndahl,David Mortimer,Christopher L. R. Barratt,Jose Antonio Castilla,Roelof Menkveld,Ulrik Kvist,Juan G. Alvarez,Trine B. Haugen No hay ninguna vista previa disponible - 2010 |
Términos y frases comunes
abnormal acrosome reaction aliquot Androl andrology andrology laboratory antibodies antisperm antibodies ASABs assay azoospermia Calculations and results Calibration capacitation CASA cells centrifugation cervical mucus chromatin clinical container counted coverslip cryobank cryopreservation dilution Disposable materials donor ejaculate epididymis equipment evaluation factor Fertil Steril fluid fructose gametes hemocytometer Hum Reprod human semen human sperm human spermatozoa hyperactivation ICSI in-vitro infertility insemination Interpretation guidelines layer leukocytes liquefied liquid nitrogen magnification male method microscope Mortimer motile spermatozoa motility assessment normal number of spermatozoa oocyte Pasteur pipettes patient pellet performed pregnancy procedure progressively motile prostatic quality control Reagents resuspend risk semen analysis semen sample seminal plasma seminal vesicles slide smears solution specimen Sperm Buffer sperm capacitation sperm chromatin sperm concentration sperm DNA sperm function sperm morphology sperm motility sperm preparation spermatogenesis staining standard storage straws subfertility technique temperature total number tube values vitro volume zinc zona pellucida