Plant Genomes: Methods for Genetic and Physical Mapping: Methods for Genetic and Physical MappingJacques S. Beckmann, Thomas C. Osborn Springer Science & Business Media, 1992 - 250 páginas The main application of genomic markers is the mapping, diagnosis, understanding, manipulation and eventual molecular cloning of loci of medical, biologic or economic interest. These achievements are likely to have a practical impact not only in basic science, e.g. in the elucidation of the basic processes involved in a particular pathway or in retracing the history of plant speciation, but also in breeding practice. In agriculture this translates into the exploitation of this knowledge for further genetic improvement since traits of agronomic interest are often polygenic. The elucidation of the genetic basis of polygenic inheritance and the subsequent efficient utilization of this knowledge in breeding schemes require the availability of an adequate network of genetic markers spanning the entire genome. The purpose of the present manual is to provide new investigators in this area with an introduction and a basic practical state-of-the-art description of how to proceed. |
Índice
Simple plant DNA isolation procedures | 1 |
Methods of Southern blotting and hybridization | 15 |
Organelle DNA isolation and RFLP analysis | 35 |
Detection of DNA sequence variation for genome analysis | 55 |
Pulsedfield gel electrophoresis | 71 |
Physical mapping by random clone fingerprint analysis | 101 |
Construction and application of a YAC library | 141 |
Linkage analysis in human genetics | 167 |
Statistical methodologies for mapping and analysis of quantitative trait loci | 181 |
Mapping quantitative trait loci using nonsimultaneous and simultaneous estimators and hypothesis tests | 209 |
additional reflections | 239 |
247 | |
Otras ediciones - Ver todo
Plant Genomes: Methods for Genetic and Physical Mapping J.S. Beckmann,T.C. Osborn Vista previa restringida - 2012 |
Plant Genomes: Methods for Genetic and Physical Mapping J.S. Beckmann,T.C. Osborn No hay ninguna vista previa disponible - 2012 |
Plant Genomes: Methods for Genetic and Physical Mapping J S Beckmann,T C Osborn No hay ninguna vista previa disponible - 1992 |
Términos y frases comunes
Acad Sci USA alleles bands bias buffer centrifuge chloroplast chloroplast DNA clones cosmid cpDNA CTAB described detection DNA fragments DNA isolation DNA molecules EDTA effects estimates ethidium bromide FIGE fingerprinting fragment length polymorphisms gel electrophoresis gene genetic maps genetic markers gradient hybridization hypothesis tests inbred incubate individual lanes large DNA large number least-square means ligation linkage map locus membranes method mismatch missing cells molecular mtDNA Natl Acad Sci nonsimultaneous Nucleic Acids Nucleic Acids Res organelle parameters pellet physical map plate plugs polymerase primer probes Proc Natl Acad procedure progeny protocol pulse Q₂ QTL genotypes quantitative trait loci random reaction recombination restriction digestion restriction enzyme restriction fragment length Resuspend room temperature sample segregating separation Southern blotting species spheroplasts statistical telomere template Theor Appl Genet tissue tomato Tris-HCl pH tube Type vector wash YAC clones YAC libraries yeast artificial chromosome
Pasajes populares
Página 245 - Tanksley SD (1988): RFLP maps based on a common set of clones reveal modes of chromosomal evolution in potato and tomato. Genetics 120:1095-1103.