Protocols in Human Molecular GeneticsProtocols in Human Molecular Genetics highlights the tremendous advances in our ability to work on the human genome that have emerged in the past few years. The latest techniques are set forth in the clear, concise, easy-to-follow format that is the hallmark of Humana's Methods in Molecular Biology series. Nearly two-thirds of the book is devoted to describing practical procedures comprising the widest range of new methodologies in human molecular genetics, with the rest focusing on their specific experimental and clinical applications. An essential tool for everyone - whether novice or seasoned expert - involved in the rapidly growing area of human genome studies. |
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Índice
The Polymerase Chain Reaction | 1 |
Direct DNA Sequencing of Complementary DNA Amplified by the Polymerase Chain Reaction | 9 |
Direct Sequencing of PCRAmplified DNA | 21 |
Rapid DNA Sequence Analysis Using Fluorescent Labels | 29 |
Detection of Mutations in DNA and RNA by Chemical Cleavage | 39 |
Rapid Methods for Detection of Polymorphic Markers in Genomic DNA | 51 |
The Analysis of Point Mutations Using Synthetic Oligonucleotide Probes | 69 |
Detection of Mutations by the Amplification Refractory Mutation System ARMS | 77 |
Yeast ArtificialChromosome YAC Cloning Systems | 197 |
Gene Targeting for Somatic Cell Manipulation | 223 |
In Situ Hybridization of Chromosomes | 233 |
Chapter 22 DNA Fingerprinting Analysis | 255 |
DNA Fingerprinting and Forensic Medicine | 273 |
The Detection of Point Mutations in Hemoglobin Defects Using AlleleSpecific Oligonucleotide Probes | 287 |
Detection of Gene Deletions Using Multiplex Polymerase Chain Reactions | 299 |
Application of PulsedField Gel Electrophoresis to Genetic Diagnosis | 313 |
Automated Gene Detection Using the Oligonucleotide Ligation Assay | 85 |
Detection of Point Mutations by DenaturingGradient Gel Electrophoresis | 95 |
The Detection and Mapping of Point Mutations by RNase A Cleavage | 111 |
Discontinuous Polyacrylamide Gel Electrophoresis of DNA Fragments | 123 |
Extraction and Enzymatic Amplification of DNA from ParaffinEmbedded Specimens | 133 |
The Use of the Polymerase Chain Reaction in the Mapping of Human Genes Using Somatic Cell Hybrids | 141 |
The Southern Blot | 147 |
The Detection of Specific DNA Sequences by Enhanced Chemiluminescence | 159 |
PulsedField Gel Electrophoresis | 169 |
Cloning from Gels Following PulseField Gel Electrophoresis | 183 |
Molecular Diagnostics of Cancer | 327 |
The Detection of Latent Virus Infection by Polymerase Chain Reaction | 347 |
Mapping Inherited Diseases by Linkage Analysis | 365 |
Diagnosis of Genetic Disorders with Linked DNA Markers | 389 |
Software for Genetic Linkage Analysis | 403 |
Creating Animal Models of Genetic Disease | 419 |
Molecular Biology and Medicine | 437 |
| 457 | |
| 459 | |
Términos y frases comunes
1991 The Humana acrylamide agarose gel aliquots allele amplification annealing approx assay bands cDNA cells centrifuge Chapter chromosome cloning concentration containing deletion denaturing detection DGGE diagnosis digestion distilled water DNA fingerprinting DNA fragments DNA samples DNA sequence Duchenne muscular dystrophy dystrophin ethanol ethidium bromide filter gel electrophoresis gene genomic DNA gradient heteroduplex human genome Human Molecular Genetics hybridization buffer Incubate labeled Lane ligation linkage analysis loci lod score markers Mathew Copyright membrane mg/mL minisatellite mismatch mixture mMEDTA mMTris-HCl Molecular Genetics Edited muscular dystrophy nucleotide oligonucleotide PCR products pellet PFGE pipet plate point mutations polyacrylamide gel polymerase chain reaction polymorphisms prepared probe Proc recombination region restriction enzyme resuspend RFLPs room temperature screening Southern blotting specific stained stock solution stored Taq polymerase target technique template tion tissue transgenic translocation tube vector wash yeast
Información bibliográfica
| Título | Protocols in Human Molecular Genetics Volumen 9 de Methods in molecular biology, ISSN 1064-3745 |
| Editor | Christopher G. Mathew |
| Edición | ilustrada |
| Editor | Springer Science & Business Media, 1991 |
| ISBN | 1592594964, 9781592594962 |
| N.º de páginas | 461 páginas |
| Exportar cita | BiBTeX EndNote RefMan |