Protocols in Human Molecular Genetics

Portada
Christopher G. Mathew
Springer Science & Business Media, 1991 - 461 páginas
Protocols in Human Molecular Genetics highlights the tremendous advances in our ability to work on the human genome that have emerged in the past few years. The latest techniques are set forth in the clear, concise, easy-to-follow format that is the hallmark of Humana's Methods in Molecular Biology series. Nearly two-thirds of the book is devoted to describing practical procedures comprising the widest range of new methodologies in human molecular genetics, with the rest focusing on their specific experimental and clinical applications. An essential tool for everyone - whether novice or seasoned expert - involved in the rapidly growing area of human genome studies.
 

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Índice

The Polymerase Chain Reaction
1
Direct DNA Sequencing of Complementary DNA Amplified by the Polymerase Chain Reaction
9
Direct Sequencing of PCRAmplified DNA
21
Rapid DNA Sequence Analysis Using Fluorescent Labels
29
Detection of Mutations in DNA and RNA by Chemical Cleavage
39
Rapid Methods for Detection of Polymorphic Markers in Genomic DNA
51
The Analysis of Point Mutations Using Synthetic Oligonucleotide Probes
69
Detection of Mutations by the Amplification Refractory Mutation System ARMS
77
Yeast ArtificialChromosome YAC Cloning Systems
197
Gene Targeting for Somatic Cell Manipulation
223
In Situ Hybridization of Chromosomes
233
Chapter 22 DNA Fingerprinting Analysis
255
DNA Fingerprinting and Forensic Medicine
273
The Detection of Point Mutations in Hemoglobin Defects Using AlleleSpecific Oligonucleotide Probes
287
Detection of Gene Deletions Using Multiplex Polymerase Chain Reactions
299
Application of PulsedField Gel Electrophoresis to Genetic Diagnosis
313

Automated Gene Detection Using the Oligonucleotide Ligation Assay
85
Detection of Point Mutations by DenaturingGradient Gel Electrophoresis
95
The Detection and Mapping of Point Mutations by RNase A Cleavage
111
Discontinuous Polyacrylamide Gel Electrophoresis of DNA Fragments
123
Extraction and Enzymatic Amplification of DNA from ParaffinEmbedded Specimens
133
The Use of the Polymerase Chain Reaction in the Mapping of Human Genes Using Somatic Cell Hybrids
141
The Southern Blot
147
The Detection of Specific DNA Sequences by Enhanced Chemiluminescence
159
PulsedField Gel Electrophoresis
169
Cloning from Gels Following PulseField Gel Electrophoresis
183
Molecular Diagnostics of Cancer
327
The Detection of Latent Virus Infection by Polymerase Chain Reaction
347
Mapping Inherited Diseases by Linkage Analysis
365
Diagnosis of Genetic Disorders with Linked DNA Markers
389
Software for Genetic Linkage Analysis
403
Creating Animal Models of Genetic Disease
419
Molecular Biology and Medicine
437
Appendix Manuals and Data Bases
457
Index
459
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