Practical Immunoassay: The State of the ArtCRC Press, 1 jun 1984 - 328 páginas |
Índice
PROBLEMS OF IODINATION | 19 |
Assessment and storage of iodinated products | 31 |
ENZYME IMMUNOASSAY | 37 |
Preparation of enzyme labels | 45 |
Conclusions | 60 |
HIGH SENSITIVITY PULSEDLIGHT TIME | 71 |
IMMUNOASSAYS USING CHEMILUMINESCENT LABELS | 103 |
NEPHELOMETRIC METHODS | 117 |
Summary and conclusions | 248 |
References | 249 |
A PRACTICAL PRIMER | 253 |
The language and goals of assay data processing and quality control | 255 |
Counting statistics and common sense | 257 |
Calibration curvefitting | 262 |
Which of these curvefitting methods is the best? | 276 |
Errors and assays | 278 |
LABELED ANTIBODY IMMUNOASSAYS | 179 |
MONOCLONAL ANTIBODIES | 199 |
Applications of monoclonal antibodies | 208 |
References | 212 |
THEIR PHYSIOLOGICAL SIGNIFICANCE AND MEASUREMENT | 217 |
The role of serumbinding proteins | 219 |
Measurement of free hormones in blood | 227 |
Standardization and quality control | 246 |
Which of these assays is superior? | 284 |
Assay design | 287 |
What does this Scatchard plot mean? | 290 |
Conclusions and recommendations | 292 |
| 295 | |
| 309 | |
Términos y frases comunes
Acta affinity analysis analyte concentration anti antibody-antigen antigen antigen concentration antigen excess antisera antiserum assay sensitivity assayist automated binding proteins Biochem biological buffer calibration curve cells centrifugal chelates Chem chemiluminescence Chim chloramine-T Clin Clinical Chemistry complexes conjugate counting curve-fitting cuvette detection developed dilution Ekins end-point Endocrinol enzyme activity enzyme immunoassay enzyme labels enzyme-linked equilibrium FIGURE fluorescence fluoroimmunoassay fluorophore fraction free hormone concentration free thyroxine hapten Hellsing human hybridomas immuno immunoadsorbent immunoglobulins immunoradiometric assay incubation instrument iodination kinetic Kohen labeled antibody laboratory laser ligand light scattering light-scattering linear luminol McAb measurement method methotrexate molecular molecules monoclonal antibodies nephelometric assays optimal particles peak rate phase plasma precipitation present problem procedure protein quality control quantitation radioimmunoassay random error reaction reagent Rodbard sample serum solid-phase antibody standard steroid techniques thyroxine tion tube two-site assay values Whicher Woodhead
Pasajes populares
Página 299 - Selection and interpretation of diagnostic tests and procedures. Principles and applications.
Referencias a este libro
Transformation and Weighting in Regression Raymond J. Carroll,David Ruppert Vista previa restringida - 1988 |