Placenta and Trophoblast: Methods and Protocols, Volume IIMichael J. Soares, Joan S. Hunt Springer Science & Business Media, 2 feb 2008 - 452 páginas The aim of the two-volume set of Placenta and Trophoblast: Methods and Protocols is to offer contemporary approaches for studying the biology of the placenta. The chapters contained herein also address critical features of the female organ within which the embryo is housed, the uterus, and some aspects of the embryo–fetus itself, particularly those of common experimental animal models. In keeping with the organization used effectively in other volumes in this series, each chapter has a brief introduction followed by a list of required items, protocols, and notes designed to help the reader perform the expe- ments without difficulty. In both volumes, sources of supplies are given and illustrations highlight particular techniques as well as expected outcomes. A key aspect of these volumes is that the contributors are at the forefronts of their disciplines, thus ensuring the accuracy and usefulness of the chapters. Placenta research has progressed rapidly over the past several decades by taking advantage of the technical advances made in other fields. For example, the reader will note that many techniques, such as reverse transcriptase po- merase chain reaction, northern and western blotting, microarray analyses and in situ hybridization experiments, are routinely used for dissecting a wide range of experimental questions. Protein analysis and functional experiments on tissues and cells that comprise the maternal–fetal interface benefit from studies in en- crinology, immunology, and developmental biology. These volumes also present new ideas on investigating gene imprinting and gene transfer via viral vectors. |
Índice
3 | |
In Vivo Analysis of Trophoblast Cell Invasion in the Human | 11 |
An In Vitro Model of Trophoblast Invasion of Spiral Arteries | 59 |
In Vivo Models for Studying Homing and Function | 77 |
Methods for Isolation of Cells from the Human | 109 |
and Placental Macrophages | 123 |
Methods for Evaluating Histocompatibility Antigen Gene | 165 |
mRNAs and Proteins | 181 |
Placental Transporters | 285 |
Analysis of Trophoblast Giant Cell Steroidogenesis | 301 |
Establishment of an ELISA for the Detection of Native Bovine | 321 |
Bacterial Expression of Prolactin Family Proteins | 341 |
Beiyan Zhou and Daniel I H Linzer 23 Methods for Studying Interferon Tau Stimulated Genes | 355 |
Reduced Uterine Perfusion Pressure RUPP Model | 383 |
In Vivo Rat Model of Preeclampsia | 393 |
A Novel Mouse Model for Preeclampsia by Transferring Activated | 401 |
In Vivo Techniques for Studying Fetoplacental Nutrient Uptake | 207 |
In Vitro Models for Studying Trophoblast Transcellular Transport | 225 |
In Vitro Methods for Studying Human Placental | 241 |
In Vitro Methods for Studying Human Placental | 253 |
Yan Xu Thomas J Cook and Gregory T Knipp 17 Heterologous Expression Systems for Studying | 265 |
Hypobaric Hypoxia as a Tool to Study PregnancyDependent | 427 |
Immunological Function | 435 |
443 | |
Otras ediciones - Ver todo
Placenta and Trophoblast: Methods and Protocols, Volume II Michael J. Soares,Joan S. Hunt No hay ninguna vista previa disponible - 2014 |
Términos y frases comunes
acid transport activity amino acid analysis antibody antigens apoptotic approx assay Biol biopsies blood bovine buffer cannula cDNA cell culture cell line centrifuge column concentration culture medium cytokines cytotrophoblasts decidual differentiation dilution DMEM EDTA ELISA enzyme explants expression fatty acid fetal fetus filter flask flow cytometry forceps function gene HLA-G hormone human placental hypertension immune immunohistochemistry Immunol incubation injection Invitrogen isolated leukocytes macrophages maternal membrane Methods and Protocols mice Molecular monocytes mouse NaCl NK cells Note Obstet oocytes ovine oxygen PAGs pellet perfusion Physiol pipet placental placental bed plasma plasmid plate preeclampsia pregnancy prepared protein radiolabeled reagents receptor Reprod Resuspend room temperature RPMI samples sections serum Sigma slides solution spiral arteries staining sterile studies substrate supernatant syncytiotrophoblast tion tissue transfected trophoblast trophoblast cells trophoblast invasion tube umbilical uptake uterine uteroplacental uterus vascular vessel villous vitro wash µg/mL