Laboratory Methods for the Detection of Mutations and Polymorphisms in DNACRC Press, 24 ene 1997 - 352 páginas The analysis of DNA sequence polymorphisms and mutations is of central importance in understanding biological systems. This book is devoted to the experimental analysis of DNA and presents easy-to-follow protocols. Various techniques from the simple to the highly complex are detailed in this volume, providing a wide spectrum of available methods and practical advice. The methods are described in terms of: This is an essential manual for researchers working in human, animal, or plant molecular genetics and is particularly valuable for hospital and commercial laboratories. |
Índice
Chapter | 1 |
6 | 46 |
7 | 55 |
8 | 66 |
9 | 90 |
10 | 99 |
Peptide Nucleic Acids | 131 |
Orphan Peak Analysis | 139 |
Enzyme Mismatch CleavageBacteriophage Resolvases | 217 |
Chemical Cleavage of Mismatch in Heteroduplexes | 225 |
Comparison of Mutation Detection by Chemical Cleavage | 237 |
Analysis of DNA Sequence Using Biosensors | 253 |
Repeat Expansion Detection | 263 |
Comparative Genomic Hybridization | 273 |
Microplate Array | 287 |
DNA Sequence Database Searches Comparisons | 307 |
Bisulphite Genomic Sequencing of Methylated Cytosines | 151 |
Heterozygote Determination Using Automated | 175 |
A Robust Radioactive Sequencing Protocol | 191 |
MutSExonuclease Protection for the Detection | 207 |
The Human Genome Database | 317 |
| 327 | |
Términos y frases comunes
Acad acrylamide agarose allele analysis annealing AP-PCR applications assay automated autoradiography bands binding biotinylated bisulphite buffer cell chromosomes cleaved cloned codon concentration cytosine databases denaturing gradient gel DGGE DNA fragments DNA polymerase DNA sequencing dNTPs endonuclease enzyme ethidium bromide exon Figure fingerprinting fluorescence gel electrophoresis gene genetic genomic DNA gradient gel electrophoresis heteroduplex heterozygote human hybridization identify Incubate labeled laboratory lane ligase ligase chain reaction ligation markers method methylation MgCl2 microsatellites minisatellite mismatch molecular mutation detection MutS MutY Natl normal Nucleic Acids Res nucleotide oligonucleotide PCR amplification PCR primers PCR products PCR reaction point mutations polyacrylamide gel polymerase chain reaction polymorphism probe Proc protein protocol purified reagents regions repeat samples screening sensitivity single base single stranded solution specific SSCP staining T4 Endonuclease VII Taq polymerase target DNA technique template tion tubes tumor wash