Molecular Biology and GenomicsElsevier, 19 jul 2010 - 272 páginas Never before has it been so critical for lab workers to possess the proper tools and methodologies necessary to determine the structure, function, and expression of the corresponding proteins encoded in the genome. Mulhardt's Molecular Biology and Genomics helps aid in this daunting task by providing the reader with tips and tricks for more successful lab experiments. This strategic lab guide explores the current methodological variety of molecular biology and genomics in a simple manner, addressing the assets and drawbacks as well as critical points. It also provides short and precise summaries of routine procedures as well as listings of the advantages and disadvantages of alternative methods.
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Acad Sci U S A activity agarose gel alkaline amplification bacteria band bases blot buffer cDNA cell lines centrifuged chromosome cloning concentration culture cycles demonstrate denatured digestion Disadvantage DNA fragments DNA polymerase DNA solution double-stranded electrophoresis ethanol exonuclease experimenter expression Figure filter fluorescence frequently functions gene genetic genome genomic DNA hybridization incubated inserted Invitrogen laboratory ligation Literature manufacture medium membrane method minutes molecular biology molecules mRNA mutation nonradioactive normal Northern blots Nucleic Acids Nucleic Acids Res nucleotides obtained oligonucleotides PCR product pellet performed phage phenol-chloroform pipetted plasmid plasmid DNA plate polymerase chain reaction possible precipitation preparation primer probes problem procedure Promega protein protocol purification quantity of DNA radioactive recombination restriction enzymes reverse transcriptase RNase Section sequences signal single-stranded specific strand Stratagene substrate suitable synthesis Taq polymerase temperature tissues transcription transfection transgenic tube usually variant vector vitro yeast
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Página 2 - Lebensglück Neuglühend mir durch Nerv' und Adern rinnen. War es ein Gott, der diese Zeichen schrieb, Die mir das innre Toben stillen, Das arme Herz mit Freude füllen, Und mit geheimnisvollem Trieb Die Kräfte der Natur rings um mich her enthüllen? Bin ich ein Gott? Mir wird so licht! Ich schau in diesen reinen Zügen Die wirkende Natur vor meiner Seele liegen.