Ovarian Cancer: Methods and ProtocolsJohn M. S. Bartlett Springer Science & Business Media, 2 feb 2008 - 817 páginas If there is one aspect of current cancer research that represents a major ch- lenge in both novice and experienced researchers, it is the rapid advance in our understanding of the disease. Researchers can be required to switch from analysis of gene expression to kinetics of protein activation, from genetic studies to the analysis of protein funtion. Cancers are highly complex disease systems and researchers aiming to understand the functioning of cancer systems require access to a wide range of laboratory techiques from a broad range of research disciplines. Increasingly, however, published methods are incomplete or refer back to a series of previous publications each containing only a small part of the complete pro- col. The aim of Ovarian Cancer: Methods and Protocols is to provide for ovarian cancer researchers in the first instance, a laboratory handbook that will facilitate research into cancer systems by providing a series of expert protocols, with proven efficacy, across a broad range of technical expertise. Thus, there are sections on tumor genetics and cellular signal transduction, as well as sections on apoptosis and RNA analysis. The value of Ovarian Cancer: Methods and Protocols to the ovarian cancer researcher will, I trust, be considerably enhanced by (1) the provision of a series of overviews relating to the biology, diagnosis, and treatment of this important neoplasm, and (2) the provision of a series of technical overviews introducing each part that provides an expert review of the applications and pitfalls of the various techniques included. |
Índice
3 | |
Alterations in Oncogenes Tumor Suppressor Genes | 37 |
Alistair R W Williams | 49 |
Tumor Markers in Screeening for Ovarian Cancer | 61 |
Primary Surgical Management of Ovarian Cancer | 75 |
Hamilton and Steven W Johnson | 89 |
An Overview | 109 |
a Prognostic Factor in Patients with Epithelial Ovarian Cancer | 121 |
for Mismatch Repair Proteins | 383 |
Karl Dobianer Michael Medl and Jürgen Spona | 389 |
A Technical Overview | 399 |
Anders Gobl and Rudi Henriksen 46 mRNA Detection by In Situ RTPCR | 411 |
Assay Analysis of mRNA for TGFβ | 431 |
RTPCR Quantitation of HSP60 mRNA Expression | 439 |
The Effects of Butyrate and the Role of cmyc in N 1 Ovarian | 449 |
Sellar Genevieve J Rabiasz Barbara Smith | 459 |
Technical Review | 145 |
Establishment of Ovarian Cancer Cell Lines | 155 |
Culture and Characterization of Human Ovarian Surface Epithelium | 169 |
Contents | 171 |
Spinner | 175 |
p53 Transfectants in Ovarian Cancer | 187 |
Cell Lines as Xenografts | 199 |
CYTOGENETICS | 207 |
Interphase Cytogenetics in Frozen Ovarian Tumor Tissue | 215 |
Susann Neubauer and Thomas Liehr 25 Rapid Identification of Chromosomes Using Primed | 223 |
Gene Amplification of cermB2 Detected by FISH | 237 |
Chromosome Microdissection for Detection of Subchromosomal | 247 |
Quantitation of FISH Signals in Archival Tumors | 253 |
Comparative Genomic Hybridization for the Analysis of Unbalanced | 261 |
A Technical Overview | 273 |
Extraction of DNA from Microdissected Archival Tissues | 291 |
RFLP Molecular Analysis of the UrokinaseType Plasminogen | 299 |
PCR Microsatellite Analysis of LOH in Ovarian Tumors | 307 |
of Loss of Heterozygosity | 315 |
in Ovarian Tumors | 323 |
p53 Deletion and Insertion Mutations | 329 |
Transfer of Human Chromosome 3 to an Ovarian Carcinoma | 337 |
Doubleand CompetitiveDifferential | 347 |
One Dimensional Genome ScanningDetection of Genomic | 357 |
Mapping of Tumor Suppressor Genes in Ovarian Cancer | 365 |
Detection of the Replication Error Phenotype in Ovarian | 375 |
PROTEIN EXPRESSION | 471 |
Measurement of IGF1 Receptor Content in Tissues | 485 |
nm23 Protein Expression by Western Blotting in Patients with Epithelial | 499 |
Simultaneous Flow Cytometric Detection of | 515 |
Quantitative Immunohistochemistry of Estrogen and Progesterone | 535 |
Quantitative Analysis of Cell Surface | 545 |
Immunoprecipitation to Determine JAK Kinase Activation in Response | 567 |
Direct Kinase Assay Screening for Inhibitors of MAP Kinase | 583 |
Induction of cfos Gene Expression by UrokinaseType | 597 |
to Lysophospholipids | 611 |
A Novel and Simple Method to Assay the Activity of Individual | 633 |
A Technical Overview | 651 |
Measurement of Apoptotic Cells by Flow Cytometry Tunnel Assay | 665 |
Use of Diphenylamine in the Detection of Apoptosis | 679 |
Differential Expression of ApoptosisAssociated Genes | 687 |
Detection of Telomerase Activity by PCRBased Assay | 701 |
A Technical Overview | 713 |
Developing AntiHER2neu SingleChain Fv Fragments | 729 |
Direction of the Recognition Specificity of Cytotoxic T Cells Toward | 749 |
Eradication of cerbB2 Positive Ovarian Cancer Cells Mediated | 757 |
Spreading of Ovarian Cancer Cells | 769 |
E1AMediated Gene Therapy | 775 |
p53 Adenovirus as Gene Therapy for Ovarian Cancer | 783 |
Bispecific Antibody MDX210 for Treatment of Advanced Ovarian | 793 |
807 | |
Otras ediciones - Ver todo
Ovarian Cancer: Methods and Protocols John M. S. Bartlett No hay ninguna vista previa disponible - 2013 |
Términos y frases comunes
acid activity adenovirus aliquot allele amplification analysis antibody antigen apoptosis assay binding blot BRCA1 BRCA2 breast cancer buffer Cancer Res cellular centrifuge chromosome cisplatin clinical clones concentration culture cytogenetic denaturation detection diluted EDTA electrophoresis enzyme epithelial ovarian cancer ethanol expression filter fluorescence formamide fragments gene genetic genomic growth factor HER2/neu hybridization Incubate interphase J. M. S. Bartlett kinase labeled markers medium membrane metaphase mg/mL mRNA mutations NaCl Northern blotting Note nuclei oncogene Oncol ovarian cancer cells ovarian carcinoma ovarian tumors overexpression patients PCR products pellet pipet plate polymerase prepared primer probe protein proteinase K reaction reagents receptor resuspend room temperature samples scFv sections sequence serum signal slides specific staining sterile store at 20°C studies Subheading supernatant technique telomerase tion tissue transfer Tris-HCl trypsin tube tumor cells tumor suppressor gene vitro wash µg/mL