Molecular Diagnostics: For the Clinical LaboratorianWilliam B. Coleman, Gregory J. Tsongalis Springer Science & Business Media, 18 abr 2013 - 390 páginas Notable practitioners describe how laboratory medicine is practiced today and illuminate how it will function tomorrow as the revolutionary advances afforded by molecular diagnostics become increasingly central to effective analysis. Proceeding from a discussion of elementary nucleic acid technology to a review of the more advanced techniques, the distinguished contributors lay the groundwork for a comprehensive understanding of their applications throughout clinical medicine. The result is a detailed description of those molecular technologies currently used in diagnostic laboratories, as well as those that seem particularly promising. Detailed discussions of specific clinical applications include those for cancer, hematological malignancies, cardiovascular disease, and neuromuscular, endocrine, and infectious diseases. |
Índice
| 3 | |
Basic Nucleic Acid Procedures | 47 |
Theory and Practice | 63 |
An Overview | 89 |
Quantitative and Diagnostic PCR in the Analysis | 103 |
PCRBased Methods for Mutation Detection | 123 |
Gene Therapy | 193 |
An Overview of Molecular Genetics | 221 |
Otras ediciones - Ver todo
Molecular Diagnostics: For the Clinical Laboratorian William B. Coleman,Gregory J. Tsongalis Vista previa restringida - 2007 |
Molecular Diagnostics: For the Clinical Laboratorian William B. Coleman,Gregory J. Tsongalis Vista previa restringida - 2006 |
Molecular Diagnostics William B. Coleman,Gregory J. Tsongalis No hay ninguna vista previa disponible - 2014 |
Términos y frases comunes
abnormalities activity allele amplification analysis annealing antigen approx assay associated BRCA1 buffer carcinoma cDNA cells cellular characterized cholesterol chromosome Clin clinical clonality cloning codon colorectal cancer concentration containing deficiency deletions denaturation detection diagnosis digestion disease disorder DNA fragments DNA probes DNA sequences exons expression factor familial gel electrophoresis gene mutations gene rearrangements gene therapy gene transfer genomic genomic DNA germline hormone human hybridization identified infection inherited intron involved labeled laboratory lane leukemia lipid lipoprotein lymphoma malignant membrane method molecular genetic molecules mRNA neoplasms normal nucleic acid nucleotide oligonucleotide patients PCR products phenotype plasmid polymerase chain reaction polymorphism primer protein proto-oncogene region resistance restriction enzyme result RET proto-oncogene reverse transcriptase RT-PCR samples segments sensitivity serum signal Southern blot specific strand syndrome synthesis techniques temperature template testing tion tissue transcription translocation Tsongalis tumor suppressor gene vectors viral virus VLDL wild-type