PCR in Bioanalysis

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Stephen J. Meltzer
Springer Science & Business Media, 1998 - 281 páginas
PCR in Bioanalysis offers powerful PCR-based protocols and assays in actual use or potential use in clinical medicine and commercial biology. The main focus of the book is on the commercial applications of PCR, as opposed to basic research uses. Topics covered include the measurement of hormone levels using PCR, transcription factor isolation, detection of viruses using PCR, detection of tumor contamination of stem cells, evaluation of grafts for tumor cells, and more.
 

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Índice

Detection of Rodent Parvoviruses by
31
Selective Genetic Analysis of Microscopic
39
Prenatal and Presymptomatic Diagnosis of Marfan Syndrome Using
49
Measurement of TNF and iNOS mRNA Using cDNAEqualized
55
PCR Diagnosis of the Bovine ImmunodeficiencyLike Virus
67
PCR Analysis for the Identification of Porcine Reproductive
81
Quantitative and Discriminative Detection of Individual HIV1 mRNA
89
Detection of the HeatLabile Toxin Coding Gene LTGene
101
Detection of Legionella Species in Bronchial Fluid by
173
Quantitative Measurement of mRNA Expression by Competitive
183
PCR Detection of Toxoplasma gondii in Human Fetal Tissues
195
Rapid Diagnosis of Pulmonary Tuberculosis Using Roche
203
The Use of Flow Cytometry and RTPCR in the Detection
215
Detection of Chlamydia trachomatis and Trichomonas vaginalis
227
Detection and Isolation of Differentially Expressed Genes by
239
Detection of Transgene Integrants and Homologous Recombinants
245

In Vitro TranscriptionTranslation Analysis for the Identification
127
A SingleTube Nested RTPCR for the Detection of Ross River Virus
145
Application of PCR to Transgenic Plants
153
Detection of Hepatitis C Virus RNA by Semiquantitative Reverse
165
Direct Analysis for Familial Adenomatous Polyposis Mutations
251
PCR Fingerprinting for Detection of Deleted or Amplified Sequences
267
Index
273
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