Nonviral Vectors for Gene Therapy: Methods and ProtocolsMark A. Findeis Springer Science & Business Media, 2 feb 2008 - 399 páginas The purpose of this volume of Methods in Molecular Medicine is to set forth examples of the great variety of techniques and applications that are now emerging in the field of nonviral gene therapy. The book emphasizes not only specific approaches to gene delivery but, in particular, the best current me- ods to prepare, handle, and characterize gene delivery agents. These topics are of very broad importance since gene therapy evolves from its mostly ac- emy-based experimental and clinical research to the ever increasing number of industry-driven programs directed toward commercial development. S- cessful introduction of nonviral gene therapy agents into the clinic should be expected to require rigorous manufacturing and analytical methods that readily meet the regulatory guidelines under which new drug candidates are reviewed for marketing approval. Exactly what those guidelines will prove to be c- tainly depends on the established guidelines for review of both biological and chemical therapeutics. Additionally, many new techniques are being devised and applied to gene therapy research; these techniques will be instrumental in developing and characterizing successful gene delivery agents. Nonviral Vectors for Gene Therapy: Methods and Protocols has two main sections. To start with, there is a series of chapters on specific protocols for the synthesis, characterization, and application of gene delivery agents. S- eral chapters address the topic of materials to bind with DNA to form the compact condensed phases that facilitate cellular delivery. |
Índice
1 | |
11 | |
Supramolecular SelfAssembly of Polyethylene glycolBlockPoly | 23 |
WaterSoluble Cationic Methacrylate Polymers for Nonviral Gene | 35 |
Stabilization of PolycationDNA Complexes by Surface Modification | 61 |
Use of Disulfide Cationic Lipids in Plasmid DNA Delivery | 79 |
Interactions of LipidOligonucleotide Conjugates with LowDensity | 89 |
Coupling of Nuclear Localization Signals to Plasmid | 105 |
Local Delivery of Therapeutic Proteins by Intratumoral Injection | 185 |
Nonviral DNA Delivery from Polymeric Systems | 195 |
Promotion of Duplex and Triplex DNA Formation by Polycation | 209 |
S Dean Allison and Thomas J Anchordoquy 19 Ultraviolet Absorption and Circular Dichroism Spectroscopy | 225 |
of Nonviral Gene Delivery Complexes | 253 |
Spectroscopy | 263 |
Sirirat Choosakoonkriang Christopher M Wiethoff | 285 |
LightScattering Techniques for Characterization of Synthetic | 349 |
and Properties Useful for Gene Delivery | 131 |
Characterization of DNA Condensates by Atomic Force Microscopy | 149 |
Rapid and Systematic Transfer and Recovery of Large BACsPACs | 159 |
Systemic Delivery of Therapeutic Proteins by Intramuscular Injection | 175 |
Renal Gene Therapy | 377 |
393 | |
Otras ediciones - Ver todo
Nonviral Vectors for Gene Therapy: Methods and Protocols Mark A. Findeis No hay ninguna vista previa disponible - 2001 |
Nonviral Vectors for Gene Therapy: Methods and Protocols Mark A. Findeis No hay ninguna vista previa disponible - 2010 |
Términos y frases comunes
agarose gel electrophoresis aggregation amino groups analysis antisense assay binding Biophys buffer cationic lipid cells charge ratio circular dichroism coating conjugates containing copolymer cytokine dendrimer determined Dissolve DNA complexes DOTAP dynamic light scattering EDTA effect endosome episomal ester ethidium bromide freeze-drying freezing gene delivery Gene Ther gene therapy gene transfer Hennink HEPES increase incubated injection interaction ligand light scattering lipid lipid-ODNs liposomes measured method mg/mL mice molecular weight molecules N:P ratio NaCl Nonviral Vectors nucleic acids obtained oleoyl oligonucleotide particles pDMAEMA pDNA pDNA-lipid peak peptide Pharm phosphate PHPMA-ONp plasma plasmid DNA polycation polycation–DNA complexes polymer polyplexes potential prepared proteins protocol renal room temperature sample serum spectra spectroscopy stability stock solution structure studies Subheading supercoiled surface synthesis targeting TBE buffer tion titration transfection transfection efficiency triplex formation tumor uptake Vectors for Gene vitro vivo µg/mL