Molecular Diagnosis of Cancer: Methods and Protocols

Portada
J. E. Roulston, John M. S. Bartlett
Springer Science & Business Media, 2004 - 393 páginas
This volume represents a diverse collection of readily reproducible methods for use in cancer detection. Highlights include FISH-based methodologies currently used in the diagnosis of solid tumors, the molecular diagnosis of genetic abnormalities by DNA array technologies-including sequence-specific oligonucleotide arrays and CGH arrays-and methodologies directed at the detection of epigenetic events and at quantitative gene expression.
 

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Índice

Prognostic and Predictive Factors
1
Assessment of Predictive Values of Tumor Markers
13
Quality Assurance of Predictive Markers in Breast Cancer
29
Extraction of Nucleic Acid Templates
59
Microdissection and Extraction of DNA From Archival Tissue
71
Fluorescence In Situ Hybridization Technical Overview
77
HER2 FISH in Breast Cancer
89
Fluorescence In Situ Hybridization for BCRABL
103
PCR Diagnosis of TCell Lymphoma in ParaffinEmbedded Bone Marrow Biopsies
209
Circulating DNA Analysis Protocols and Clinical Applications Using Taqman Assays
217
Microsatellite Instability Theory and Methods
237
Diagnostic and Prognostic Significance of the Methylation Status of Myf3 in Lymphoproliferative Disorders
251
Quantitative Analysis of PRAME for Detection of Minimal Residual Disease in Leukemia
267
Determination of Cyclin D1 Expression by Quantitative RealTime ReverseTranscriptase Polymerase Chain Reaction
277
Detection of Telomerase hTERT Gene Expression and Its Splice Variants by RTPCR
297
Detection of Telomerase Enzyme Activity by TRAP Assay
311

UroVysion Multiprobe FISH in Urinary Cytology
117
Chromogenic In Situ Hybridization in Tumor Pathology
133
Comparative Genomic Hybridization and Fluorescence In Situ Hybridization in Chronic Lymphocytic Leukemia
145
Molecular Characterization of Human Papillomaviruses by PCR and In Situ Hybridization
159
A Nested RTPCR Assay to Detect BCRabl
181
TP53 Mutation Detection by SSCP and Sequencing
191
Identification of TP53 Mutations in Human Cancers Using Oligonucleotide Microarrays
323
Detection of Kras Mutations by a Microelectronic DNA Chip
337
MicroarrayBased CGH in Cancer
355
Tissue Microarrays
377
Index
391
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