Protein Expression Technologies: Current Status and Future Trends
Advances in protein expression technologies have mushroomed in recent years. In this book current and emerging expression technologies are reviewed. Reviews of the molecular genetics of expression systems in various organisms are presented. Topics covered include: Expression of extremophilic proteins; expression in E. coli, Bacillus spp., Saccharomyces cerevisiae, and methylotrophic yeasts; insect cell expression and the baculovirus system; and Chinese Hamster Ovary (CHO) cell lines for large-scale protein production. Also covered are two emerging expression systems, Methylobacterium extorquens AM1 and Caulobacter crescentus.
Comentarios de usuarios - Escribir una reseña
No hemos encontrado ninguna reseña en los sitios habituales.
Expression Folding and Degradation
Yuchan Chao Pharmaceutical Research
Tools for Metabolic Engineering
Expression Systems in Bacillus
Production of Recombinant Proteins Using
Advances in Protein Expression
acid activity addition allow analysis antibody Bacillus subtilis bacteria Bacteriol bacteriophage baculovirus binding Biochem Biol Biotechnol cerevisiae chaperone characterization Chem chromosome cloning codon complex concentration containing copy culture cytoplasm degradation developed disulfide effect efficient encoding engineering Environ enzymes Escherichia coli et al example expression systems extremophile factor feeding fermentation Figure folding function gene expression genetic genome glucose glycosylation growth heterologous proteins host human hyperthermophilic important improved increase induced initial insect insect cells integration interactions interest involved isolated levels mammalian markers membrane metabolic methanol method Microbiol molecular mutants Nature organisms pastoris pathway peptide periplasmic Pichia plasmid present production promoter protease protein expression protein production Pyrococcus recently recombinant proteins region regulation replication result secretion selection sequence signal specific stability strains strategies structure studies substrate subtilis transcription transformation translation vector yeast