Protein Expression Technologies: Current Status and Future TrendsFrançois Baneyx Garland Science, 2004 - 532 páginas Advances in protein expression technologies have mushroomed in recent years. In this book current and emerging expression technologies are reviewed. Reviews of the molecular genetics of expression systems in various organisms are presented. Topics covered include: Expression of extremophilic proteins; expression in E. coli, Bacillus spp., Saccharomyces cerevisiae, and methylotrophic yeasts; insect cell expression and the baculovirus system; and Chinese Hamster Ovary (CHO) cell lines for large-scale protein production. Also covered are two emerging expression systems, Methylobacterium extorquens AM1 and Caulobacter crescentus. |
Índice
Expression of Extremophilic Proteins | 1 |
Expression Folding and Degradation | 85 |
Tools for Metabolic Engineering | 149 |
Expression Systems in Bacillus | 199 |
Production of Recombinant Proteins Using | 297 |
Advances in Protein Expression | 345 |
Insect Cell Expression Systems | 387 |
Heterologous Expression of Genes | 427 |
Heterologous Protein Expression | 461 |
Secretion or Presentation of Recombinant | 477 |
| 525 | |
Términos y frases comunes
Acad activity alkaliphilic amino acids analysis antibody Archaea Aspergillus Bacillus subtilis bacteria Bacteriol bacterium baculovirus Baneyx binding Biochem Bioeng Biol Biotechnol C-terminal Caulobacter cell lines cellular chaperone characterization Chem CHO cells chromosome cloning codon coli-PET system T7 copy number culture cytoplasm degradation dehydrogenase developed disulfide bond encoding enzymes Escherichia coli eukaryotic expression system expression vectors extremophile factor fermentation function fusion gene expression genetic genome glucose glycosylation growth halophilic heterologous proteins homologous host hyperthermophilic hyperthermophilic archaeon insect cells integration interactions levels mammalian cells markers membrane methanol methanolica methylotrophic Microbiol molecular mutants Natl overexpression pathway peptidase periplasmic Pichia pastoris plasmid Proc protease protein expression protein production protein secretion psychrophilic purification Pyrococcus furiosus recombinant proteins reesei regulation replication S-layer Saccharomyces cerevisiae selection signal peptide signal sequence solfataricus stability strains structure substrate subunit Sulfolobus system T7 promoter thermophilic Thermotoga maritima transcription transformation translocation virus yeast